Proteins were subsequently separated on 12% (wt/vol) polyacrylamide gels or Mini-Protean TGX Any kD gradient gels (Bio-Rad), transferred to PVDF membranes (GE Healthcare), blocked with PBS answer in addition 2% (vol/vol) BSA, and probed with commercially available monoclonal antibodies to tetherin (clone RS38E), NTB-A (clone NT-7), CD4 (clone mAb51312), -actin (clone ACTN05), HIV-1 Env (clone 1994), HIV-1 Gag (clone 3A1), or a rabbit polyclonal antibody to Vpu obtained through the ARP from Klaus Strebel (NIAID, NIH) (51)
Proteins were subsequently separated on 12% (wt/vol) polyacrylamide gels or Mini-Protean TGX Any kD gradient gels (Bio-Rad), transferred to PVDF membranes (GE Healthcare), blocked with… Read More »Proteins were subsequently separated on 12% (wt/vol) polyacrylamide gels or Mini-Protean TGX Any kD gradient gels (Bio-Rad), transferred to PVDF membranes (GE Healthcare), blocked with PBS answer in addition 2% (vol/vol) BSA, and probed with commercially available monoclonal antibodies to tetherin (clone RS38E), NTB-A (clone NT-7), CD4 (clone mAb51312), -actin (clone ACTN05), HIV-1 Env (clone 1994), HIV-1 Gag (clone 3A1), or a rabbit polyclonal antibody to Vpu obtained through the ARP from Klaus Strebel (NIAID, NIH) (51)