The diversity of each unpanned library was determined by random sampling of ~20 phage clones, phagemid extraction with a plasmid preparation system (QIAGEN), and Sanger sequencing for VH/VL-gene usage and VH/L-CDR3 sequences (obtained sequences were subjected to an online database search at IMGT/V-QUEST and vbase2, accessible at http://www
The diversity of each unpanned library was determined by random sampling of ~20 phage clones, phagemid extraction with a plasmid preparation system (QIAGEN), and Sanger… Read More »The diversity of each unpanned library was determined by random sampling of ~20 phage clones, phagemid extraction with a plasmid preparation system (QIAGEN), and Sanger sequencing for VH/VL-gene usage and VH/L-CDR3 sequences (obtained sequences were subjected to an online database search at IMGT/V-QUEST and vbase2, accessible at http://www