A fragment from the chitin synthase transcript was amplified by PCR using Seeing that-5-CGACACACACAATCCCTGTC-3 and S-5-TTCGTCACTCAAGGCTGTTG-3 oligonucleotides, cloned into TOPO vectors (Invitrogen) and riboprobes were generated with T3 and T7 polymerases based on the manufacturer’s protocols
A fragment from the chitin synthase transcript was amplified by PCR using Seeing that-5-CGACACACACAATCCCTGTC-3 and S-5-TTCGTCACTCAAGGCTGTTG-3 oligonucleotides, cloned into TOPO vectors (Invitrogen) and riboprobes were… Read More »A fragment from the chitin synthase transcript was amplified by PCR using Seeing that-5-CGACACACACAATCCCTGTC-3 and S-5-TTCGTCACTCAAGGCTGTTG-3 oligonucleotides, cloned into TOPO vectors (Invitrogen) and riboprobes were generated with T3 and T7 polymerases based on the manufacturer’s protocols